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Fig. 2 fz8a function is not required for dorso-ventral patterning of the spinal cord. A, B: Tg(hsp70:fz8aCRDTM-egfp) embryos were heat shocked at 18 hpf. A: Control embryos had high levels of EGFP-fluorescence in the whole embryos. B: Embryos injected with fz8a MO had low levels of EGFP fluorescence, suggesting that fz8a MO works specifically to knock-down fz8a expression. C-F: Transverse sections of the spinal cord of Tg(olig2:egfp) embryos. C, E: Control embryos that show olig2-EGFP and iro3 expression (C), or olig2-EGFP and nk2.2 expression (E), revealed by in situ RNA hybridization. D, F: Tg(olig2:egfp) embryos injected with fz8a MO and hybridized by iro2 (D) and nk2.2 (F) RNA probes.