IMAGE

Fig. S3

ID
ZDB-IMAGE-081104-1
Genes
Source
Figures for Leung et al., 2008
Image
Figure Caption

Fig. S3 Translational blocking by gpr161-MO morpholino detected by in vitro cell lysate assay. (A) Two translational blocking morpholinos, targeted against the 5′UTR upstream of the ATG start codon of zebrafish gpr161 open-reading frame, which was fused to V5-6xHistidine epitope. (B,C) Dose dependent inhibition of Gpr161 translation by two independent gpr161 morpholinos (gpr161-MO#36 and gpr161-MO#24) using in vitro cell lysate. No inhibition by 5-base mismatch control morpholino (mismatch-MO#28). Anti-V5 antibody was used to detect the in vitro translation (B) of Gpr161-V5 fusion protein and band intensity (C) was quantitated as % translation compared to control in the graph. No morpholino as control (100%), 10 μM MO#24 (7%), 1 μM MO#24 (56%), 0.1 μM MO#24 (84%), 10 μM MO#28 (79%), 1 μM MO#28 (92%), 0.1 μM MO#28 (79%), 10 μM MO#36 (0%), 1 μM MO#36 (13%) and 0.1 μM MO#36 (40%). (D) Both gpr161 morpholinos (MO#24 and MO#36) gave similar cardiac looping defect. All scale bars were 100 μm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 323(1), Leung, T., Humbert, J.E., Stauffer, A.M., Giger, K.E., Chen, H., Tsai, H.J., Wang, C., Mirshahi, T., and Robishaw, J.D., The orphan G protein-coupled receptor 161 is required for left-right patterning, 31-40, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.