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Fig. 5 Expression analysis of T/XEX-GM2′2. Progeny from a homozygous transgenic female outcross (A, B) and a homozygous transgenic male outcross (C, D, E–H, J, L) are compared with wt embryos (I, K). Embryos were analyzed at the late sphere stage (A–D); 3- to 4-somite (E, F); 24 hpf (G, H); 48 hpf (I, J); 5 dpf (K, L), and homozygous adults (N) were compared with wt adults (M) for GFP expression. Images were captured under brightfield (A, C, E, G) and a bandpass GFP filter set (B, D, F, H, I, J, K, L, M, N).
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Reprinted from Developmental Biology, 263(2), Davidson, A.E., Balciunas, D., Mohn, D., Shaffer, J., Hermanson, S., Sivasubbu, S., Cliff, M.P., Hackett, P.B., and Ekker, S.C., Efficient gene delivery and gene expression in zebrafish using the Sleeping Beauty transposon, 191-202, Copyright (2003) with permission from Elsevier. Full text @ Dev. Biol.