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Figure Caption
Fig. 4 Ubiquitous expression of GFP, BFP and DsRed from transposon vectors. A schematic representation of each transposon used to generate the transgenic lines (Tg) is located above each set of fluorescent embryos in (A), (D), and (G). The T2-based vector expressing GFP is under the control of s1EF1α (A), and the T-based vectors expressing DsRed-1 (D) and BFP (G) are under the control of EF1α enhancer/promoter (XEX). Multiple generations expressing GFP (B, C), DsRed (E, F), and BFP (H, I) are shown at 25 hpf (B, E, H) and 5 dpf (C, F, I) along with a wild-type (wt) embryo.
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Reprinted from Developmental Biology, 263(2), Davidson, A.E., Balciunas, D., Mohn, D., Shaffer, J., Hermanson, S., Sivasubbu, S., Cliff, M.P., Hackett, P.B., and Ekker, S.C., Efficient gene delivery and gene expression in zebrafish using the Sleeping Beauty transposon, 191-202, Copyright (2003) with permission from Elsevier. Full text @ Dev. Biol.