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Fig. 1

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ZDB-IMAGE-081021-22
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Figures for Davidson et al., 2003
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Figure Caption

Fig. 1 Injection scheme for the generation of SB transgenic lines. (A, left) Structure of a Sleeping Beauty transposon consisting of flanking IR/DR sequences (gray with orange triangles), a ubiquitous EF1α enhancer/promoter (blue) driving expression of a GFP reporter (green), and terminating with a SV40 poly(A) signal (white). In vitro-transcribed, capped SB10 transposase mRNA. The SB transposon is injected with or without SB10 mRNA into one-cell zebrafish embryos (A, middle), resulting in mosaic GFP expression at 24 hpf (A, far right). (B, left) Each injected transposon vector +/- SB10 mRNA is listed with the corresponding germline transmission and expression frequency, obtained as the percentage of F0 fish producing GFP-positive offspring. The number of fish screened is shown in parentheses. The F0 germline mosaicism rate is determined as the percentage of GFP-positive F1 offspring from a founder outcross. The number of linkage groups harboring a transposon insertion is also listed. For each experiment, embryos with germline integration of ubiquitous GFP expression at 25 hpf in the F2 (top and bottom) and F8 (middle) generations are shown under GFP fluorescence detection conditions.

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Reprinted from Developmental Biology, 263(2), Davidson, A.E., Balciunas, D., Mohn, D., Shaffer, J., Hermanson, S., Sivasubbu, S., Cliff, M.P., Hackett, P.B., and Ekker, S.C., Efficient gene delivery and gene expression in zebrafish using the Sleeping Beauty transposon, 191-202, Copyright (2003) with permission from Elsevier. Full text @ Dev. Biol.