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Fig. 1 Examples of different classes of transgenic zebrafish. (A) Unrestricted expression of nuclear-targeted GFP in a 24-h postfertilization (hpf) ef1αGFPntd embryo. (B) Dorsolateral view of hindbrain of a 24-hpf ef1αYFPntd embryo. (C) Neuron-restricted expression of YFP in a 24-hpf HuC/YFP embryo. (D) Ventral view of eyes and forebrain from a HuC/YFP 24-hpf embryo. (E) YFP expression is predominantly in the rostral third of the developing spinal cord in a 31-hpf RAREGT2/YFP embryo. (F) YFP expression is expanded both rostrally into the brain and caudally within the spinal cord in a 31-hpf RAREGT2/YFP embryo exposed to retinoic acid (10-6 M for 6 h beginning at the 19- to 20-somite stage). (G) No GFP expression is observed in a 30-hpf zfHSP/GFP embryo grown at 28.5°C. (H) GFP expression is rapidly induced in a 30-hpf zfHSP/GFP embryo following exposure to 37°C for 1 h (beginning at 24 hpf). All embryos, with the exception of (D), are lateral view with rostral right and dorsal up. The HuC promoter was a gift from Dr. A.B. Chitnis. The HSP/GFP fish were a gift from Dr. J.Y. Kuwada.
Reprinted from Developmental Biology, 256(1), Udvadia, A.J. and Linney, E., Windows into development: historic, current, and future perspectives on transgenic zebrafish, 1-17, Copyright (2003) with permission from Elsevier. Full text @ Dev. Biol.