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Figure Caption
Fig. 3 Autoregulation of bozozok prior to the onset of gastrulation. nieuwkoid/dharma RNA is present in wild-type embryos (a) but not in homozygous bozm168 embryos (b) as detected by in situ hybridization at sphere stage (4 hpf). (c) Post-in situ genotyping of embryos in (a) and (b) using PCR and digestion with BspHI. Lane 1, size marker; lane 2, homozygous wild-type embryo (*AB strain) shown in (a); lane 3, homozygous bozm168 embryo shown in (b); lane 4, no-template control.
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Reprinted from Developmental Biology, 215(2), Koos, D.S. and Ho, R.K., The nieuwkoid/dharma homeobox gene is essential for bmp2b repression in the zebrafish pregastrula, 190-207, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.