ZFIN Image: Yoshida et al., 2008, Fig. 2

Image

Figure Caption

Fig. 2 Localization of zebrafish Il1rapl1b tagged with EYFP in developing zebrafish olfactory sensory neurons. (A) Omp promoter-driven expression vectors for EYFP-Il1rapl1b fusion protein and VAMP2-ECFP (Pomp-VC-Y-Il1rapl1b, top) and for Il1rapl1b-EYFP-fusion protein and VAMP2-ECFP (Pomp-VC-Il1rapl1b-Y, bottom) in olfactory sensory neurons. Black boxes, the omp promoter; crosshatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, pBluescript II SK+. Schematized structures of EYFP-tagged Il1rapl1b proteins are on the right. (B) Representative VAMP2-ECFP signals (left, green) and EYFP-Il1rapl1b signals (middle, red) in olfactory sensory neurons of Pomp-VC-Y-Il1rapl1b-injected embryos at 60 hpf. These signals are merged on the right. op, olfactory placode; ob, olfactory bulb. Arrows and arrowheads point cell bodies and axon terminals, respectively. Bar = 20 μm. (C, D) Representative VAMP2-ECFP signals (left, green) and EYFP-fusion protein signals (middle, red) in the axon terminal of olfactory sensory neuron of Pomp-VC-Y-Il1rapl1b (C)-or Pomp-VC-Il1rapl1b-Y (D)-injected embryos at 60 hpf. These signals are merged on the right. Arrowheads point axonal shafts. Bar = 5 μm.

Acknowledgments

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Reprinted from Molecular and cellular neurosciences, 39(2), Yoshida, T., and Mishina, M., Zebrafish orthologue of mental retardation protein IL1RAPL1 regulates presynaptic differentiation, 218-228, Copyright (2008) with permission from Elsevier. Full text @ Mol. Cell Neurosci.