Fig. 1

Fig. 1 Rohon Beard cell generation and loss during early development. (A–C) HNK-1-labeled RB cells in spinal cords of zebrafish embryos at 15 s (A), 26 hpf (B), and 32 hpf (C). Dorsal views with anterior up. Scale bar, 50 μm. (D and E) Anti-Hu-labeled DRG at 48 (D) and at 60 hpf (E). At 48 hpf, only a small number of neurons (arrows) are labeled in the most anterior DRG, whereas by 60 hpf, one or two neurons are labeled in each of the DRG segmentally positioned along the trunk (arrows). Lateral views with anterior to the left. (F) Graph showing the total number of HNK-1⁺ differentiated RB cells (red), the number of apoptotic (TUNEL-labeled) RB cells (blue), and the number of trkC1-positive RB cells (yellow) per embryo over time. Values represent means ± SEM. Arrow indicates the stage at which DRG neurons start to differentiate.