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Fig. S1 RTPCR reveals vegetal marker presence in early (A) and late (B) stage oocytes in wild-type and buc (mut). C) QRTCPR shows that the relative expression levels of bruno-like and mago nashi transcripts are similar in wild-type and mutant oocytes corroborating the notion that these vegetal mRNAs are present but not localized in bucky ball mutant oocytes.
                
                    
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                        Acknowledgments
                    
                    
                
                
            
        
        
    
        
            
            
                
                    
                        
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    Reprinted from Developmental Biology, 321(1), Marlow, F.L., and Mullins, M.C., Bucky ball functions in Balbiani body assembly and animal-vegetal polarity in the oocyte and follicle cell layer in zebrafish, 40-50, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.
 
        