ZFIN Image: Douglass et al., 2008, Fig. 1

Image

Figure Caption

Fig. 1 Photoactivation of ChR2 in Zebrafish Somatosensory Neurons Triggers Escape Behaviors

(A) Lateral view of a 24 hpf embryo expressing ChR2-YFP and EGFP in touch-sensitive Rohon-Beard and trigeminal neurons. Anterior is at left, and dorsal is at top. The scale bar represents 100 μm.

(B) Maximum-intensity Z projections of two-photon stacks showing ChR2-YFP and EGFP expression in trigeminal (top panel) and Rohon-Beard (bottom panel) neurons at 24 hpf. The scale bar represents 10 μm.

(C) Time-series projection of a ChR2-YFP-expressing embryo performing an escape in response to illumination at 488 nm. Images were acquired at 500 fps. The scale bar represents 100 μm.

(D) Percentage of experimental (Isl1::Gal4-VP16::UAS-E1b::ChR2-YFP, UAS::GFP) and control (Isl1::Gal4-VP16, UAS::GFP) embryos showing light-evoked escape behaviors. Data are mean ± SEM across three (ChR2) and two (control) clutches of 50–100 injected embryos. Experimental: 79% ± 4%, n = 149 embryos; control: 1% ± 1%, n = 66 embryos.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.