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Fig. 7

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ZDB-IMAGE-080828-22
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Figures for Goessling et al., 2008
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Fig. 7 Liver regeneration following partial hepatectomy in adult zebrafish is enhanced by wnt activation. (A–D) En-bloc dissection of APC+/- and hs:wnt8 (induced from 6 to 18 hpr) fish at 3 dpr revealed enhanced regeneration, while inhibition of signaling by dnTCF (induced from 6 to 18 hpr) resulted in severely impaired regenerative capacity. (E) Quantitative analysis of the liver lobe:remnant ratios in zebrafish at days 1, 3, 5 and 7 post resection reveals enhanced regenerative kinetics caused by wnt/β-catenin activation in APC+/- (blue triangle) and wnt8 (light blue diamond) fish, while dnTCF (green circle) inhibits liver regeneration. (F) In vivo volume measurements of the inferior liver lobe by high-frequency ultrasound at 3 dpr confirmed the regenerative advantage in APC+/- (blue) and wnt8 (light blue) fish and the decreased regenerative capacity in dnTCF fish (green) (ANOVA, n = 3/genotype, p < 0.05). (G–J) IHC in these fish at 3 dpr revealed increased cytoplasmic and nuclear β-catenin staining in APC+/- and hs:wnt8 fish. (K–N) Hepatocyte proliferation measured by PCNA staining was increased in APC+/- and wnt8 fish, but absent in dnTCF fish. Significant differences are indicated with an asterisk (∗).

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Reprinted from Developmental Biology, 320(1), Goessling, W., North, T.E., Lord, A.M., Ceol, C., Lee, S., Weidinger, G., Bourque, C., Strijbosch, R., Haramis, A.P., Puder, M., Clevers, H., Moon, R.T., and Zon, L.I., APC mutant zebrafish uncover a changing temporal requirement for wnt signaling in liver development, 161-174, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.