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Fig. 3 Confirmation of transgenic fish by Southern analysis. Pst I digests of genomic DNA were subjected to Southern analysis by hybridizing with a probe from the lacZ gene (generated by using primers PC17 and PC18 in Fig. 1). Comparisons of hybridizing band patterns between the offspring pools of F1 transgenic fish (lanes 1-6) and 30 pg of the pRSV-βGal plasmid (lane 7), equivalent to ≈1.34 copies per diploid genome. Genomic zebrafish DNA in lanes: 1, F2-45; 2, F9-45; 3, F17-114; 4, M14-5; 5, M9-51; 6, M9-37; 8, offspring pool from a nontransgenic F1 fish derived from transgenic fish F17.
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