ZFIN Image: Lin et al., 1992, Fig. 6

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Figure Caption

Fig. 6 Southern analysis of DNA from pigmented and albino offspring of chimera 5-5-3 showing inheritance of the transgene in some pigmented offspring. Pst I digests of genomic DNA from individual fish were analyzed as described under Materials and Methods. Lane 1, albino fish DNA to which was added 50 pg of the RSV-lacZ plasmid, equivalent to approximately two copies per diploid genome; lane 2, albino fish DNA; lane 3, DNA from a heterozygous M9-1 donor transgenic fish; lanes 4-8, DNA from five individual pigmented offspring of chimera 5-5-3, three of which inherited the lacZ gene; lanes 9-11, DNA from three individual albino offspring of chimera 5-5-3, none ofthem carried the lacZ gene. kb, Kilobase(s).

Acknowledgments

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