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Fig. 7 RT-PCR analysis of Di-cis constructs. (A) Schematic view of the Di-cis constructs used for the RT-PCR analysis including the relative position of the haipin structure (HP), the chimeric human globin intron (137 nt), and the primers used for RT-PCR analysis indicated as arrows (see Additional file 5). B) RT-PCR from N2A cells transiently transfected with the control vector pRF-Di cis and the pRF-IR2 construct. A 1 kbp DNA ladder is shown on the left side of the gel. NTC, denotes no template control, PCR products generated from plasmid DNA are denoted as plasmid. RT-PCR products are indicated as cDNA. The 18s rRNA control PCR served as internal standard as described previously (49)