ZFIN Image: Jessen et al., 1998, Fig. 2

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Figure Caption

Fig. 2 Southern blot analysis of three different GATA-2 BAC clones modified by the G2TC DNA fragment. Newly introduced restriction sites (Fig. 1D) were used to digest both wild-type (lanes 1) and modified (lanes 2) BAC DNA. (A) GATA-2 BAC clone 3 was digested with the indicated enzymes and probed with a 2-kb wild-type ClaI-EcoRV genomic fragment (Fig. 1B). A 9-kb wild-type EcoRV fragment became 8.8-kb and 2-kb fragments. An 11-kb wild-type XhoI-BamHI fragment became 9-kb and 2-kb fragments. A 20-kb wild-type SalI fragment became 12-kb and 8.6-kb fragments. A 10.5-kb wild-type ClaI fragment became 9.5-kb and 1.7-kb fragments. A 13.5-kb wild-type HindIII fragment became 12-kb and 2.5-kb fragments. GATA-2 BAC clones 1 and 4 (B) also contained the desired gene replacement.

Acknowledgments

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