Fig. 4

Fig. 4 The ntl phenocopy produced by microinjection of double-stranded Zf-T RNA into single-cell embryos. (A) No abnormal phenotypes were apparent subsequent to injection of Zf-T sense or antisense RNA. The injection of double-stranded control RNA also produced no apparent phenotypes. Embryos injected with double-stranded Zf-T RNA exhibited greatly reduced tails and their somites lacked the typical chevron shape of those in the wild-type embryos. The embryos shown are 5 days old (A). (B) In situ hybridizations demonstrate that 9-h embryos lack Zf-T expression following double-stranded Zf-T RNA injection into single-cell embryos, although sense RNA, antisense RNA, and doublestranded control RNA injection had little effect on Zf-T expression. Scale bars = 100 μm.