IMAGE

Fig. 4

ID
ZDB-IMAGE-080613-4
Genes
Source
Figures for Asakawa et al., 2008
Image
Figure Caption

Fig. 4 Expression of TeTxLC:CFP in double transgenic embryos. (A) The UAS:TeTxLC:CFP fish carries a single-copy insertion of T2SUASTeTxLCCFP within the CSPP1 gene. Blue boxes indicate exons. (B-G) Lateral views of the trunk of double transgenic embryos immunostained with the anti-GFP antibody (green) and the anti-Hb9 (B-E) or the zn-12 (F and G) antibody (red). Arrowheads indicate costaining with anti-GFP and anti-Hb9 (E) or anti-GFP and zn-12 (G). Anterior is to the left, and dorsal is to the top. (Scale bars: 50 µm.) (B and C) The hspGGFF27A;UAS:TeTxLC:CFP embryo at 48 hpf. The anti-GFP antibody detects the TeTxLC:CFP fusion protein but does not detect the GGFF protein in this condition (data not shown). Axons of descending hindbrain interneurons were strongly stained (green). The anti-Hb9 antibody detected spinal motor neurons (red). (D and E) The SAGFF31B;UAS:TeTxLC:CFP embryo at 48 hpf. The anti-GFP antibody detected spinal interneurons and motor neurons (green). (F and G) The SAGFF36B;UAS:TeTxLC:CFP embryo at 30 hpf. Both the anti-GFP and zn-12 antibodies detected Rohon-Beard neurons (green and red).

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA