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Fig. 6 Light induces gene expression during the subjective night. RNase protection analysis of Per1, Per2, Per3, Clock, Bmal1, and Bmal2 gene expression in Z3 cells entrained for 5 days under a 12:12 LD cycle and then given a light pulse. The light pulse began at ZT16 and lasted for 5, 15, 30, 60, and 120 min. RNA was harvested at each time point for light pulsed (L) and nonlight pulsed (D) samples. tRNA serves as a negative control reaction (t). Relative amounts of total RNA used for each sample are displayed (RNA).
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