ZFIN Image: Lyons et al., 2002, Fig. 5

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Figure Caption

Fig. 5 DNA-binding activity of Gata1 and Gata1 (Arg-339 → STOP). (a) Unlabeled in vitro translated proteins, wild-type zebrafish Gata1 (zgata1), zebrafish Gata1 (Arg-339 → Stop) (Arg-Stop), and negative control protein, luciferase (luc), were used in an electrophoretic mobility shift assay with the indicated ³²P-labeled probes, β/ε globin or α1-globin. The DNA-protein complexes are seen as slower migrating bands, marked by an arrowhead. The arrow marks free probe, demonstrating excess of DNA in the mixture. (b) Polyacrylamide gel electrophoresis of in vitro translated proteins generated in the presence of [³⁵S]methionine and visualized by autoradiography.

Acknowledgments

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