ZFIN Image: Dupuy et al., 2002, Fig. 1

Image

Figure Caption

Fig. 1 Verifying activity of SB10 mRNA. In vitro-transcribed SB10 mRNA was injected into zebrafish embryos homozygous for a transposon insertion (line E). Embryos are then collected after 24 h, and genomic DNA is extracted from them (a). PCR is then performed by using primers that flank the site of transposon insertion. Transposition can then be detected by appearance of the PCR product from the transposon insertion site after excision (b). Excision can be confirmed by sequencing the PCR product to detect the three nucleotide footprint (c). The sequence of the insertion site following transposon excision is indicated (*). Two different base-pair footprints are found in roughly equal proportion, CAG or CTG.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA