IMAGE

Fig. 3

ID
ZDB-IMAGE-080529-51
Source
Figures for Taylor et al., 2004
Image
Figure Caption

Fig. 3 Biochemical characterization of noa. (A) Immunoblots of porcine heart PDH (control), wild-type noaa13 siblings [wt (noaa13)], noaa13 mutants (noaa13), wild-type noam631 siblings [wt (noam631)], noam631mutants (noam631), and embryos at 1 hpf using anti-Dlat (Upper) or anti- glial fibrillary acidic protein as a loading control (Lower). (B Upper) RT-PCR (cDNA) and genomic DNA (DNA) PCR using primers that flank a small intron showing mRNA is maternal. (B Lower) PCR using primers that flank the C to T transition in the a13 allele followed by restriction digestion with NlaIII. Quantification of lactate (wt, 165 ± 12 pmol per larvae; noa, 2,655 ± 2 pmol per larvae) (C); pyruvate (wt, 101 ± 7 pmol per larvae; noa, 267 ± 13 pmol per larvae) (D); and ATP and ADP (ATP: wt, 65 ± 1 pmol per larvae; noa, 65 ± 1 pmol per larvae; and ADP: wt, 5.8 ± 0.6 pmol per larvae; noa, 5.9 ± 0.3 pmol per larvae) (E)at 6 dpf are shown. Graphs indicate relative values, and the error bars are the SEM.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA