ZFIN Image: Hashimoto et al., 2000, Fig. 4

Image

Figure Caption

Fig. 4 Overexpression of dkk1 RNA results in an enlargement of the forebrain and axial mesendoderm. (A) The embryos injected with 25 pg of dkk1 RNA at 6 hpf (shield stage) exhibited an expanded expression domain of dorsal mesendodermal markers (right panels), compared to an uninjected embryo (left panels). din, chordin; gsc, goosecoid; axl, axial; ntl, no tail. (B) In embryos injected with dkk1 RNA (right panels) at late-gastrula stages (90% epiboly), expression domains of anterior neuroectoderm markers, six3 and otx2, as well as prechordal mesendoderm markers, hgg1 and gsc, were enlarged compared to uninjected siblings (left panels). In contrast, expression of pax2.1 and fgf8, observed in prospective MHB of control embryos, was not detected in embryos injected with dkk1 RNA.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 217(1), Hashimoto, H., Itoh, M., Yamanaka, Y., Yamashita, S., Shimizu, T., Solnica-Krezel, L., Hibi, M., and Hirano, T., Zebrafish Dkk1 functions in forebrain specification and axial mesendoderm formation, 138-152, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.