ZFIN Image: Hashimoto et al., 2000, Fig. 3

Image

Figure Caption

Fig. 3 Effects of Dkk1 overexpression on zebrafish development. The embryos injected with 25 pg of dkk1 RNA were analyzed by microscopic observation at 13 hpf (A), 24 hpf (B), and 3 dpf (C). Typical phenotypes are shown. (A) Uninjected (left panel) and dkk1 RNA-injected embryos (middle and right) at 13 hpf (the 8-somite stage for wild-type embryos). Dorsal views. Note expansion of notochord and reduction and malformation of somites in the injected embryos, compared to uninjected embryos. In severely affected embryos (right), somites were not apparent. (B) Uninjected (left) and dkk1 RNA-injected embryos (right) at 24 hpf. Lateral views. The injected embryos had enlarged heads and eyes and shortened anterior-posterior axes. (C) Uninjected (top) and dkk1 RNA-injected embryos (middle, mildly affected embryo; lower, severely affected embryo) at 3 dpf. Lateral views.

Acknowledgments

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Reprinted from Developmental Biology, 217(1), Hashimoto, H., Itoh, M., Yamanaka, Y., Yamashita, S., Shimizu, T., Solnica-Krezel, L., Hibi, M., and Hirano, T., Zebrafish Dkk1 functions in forebrain specification and axial mesendoderm formation, 138-152, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.