ZFIN Image: Chiang et al., 2001, Fig. 9

Image

Figure Caption

Fig. 9 Binding of Sox9a and Sox9b to specific DNA sequences. (A) The sequences of oligo used in the protein–DNA-binding experiment. The sequence of the known binding site for SOX9 is underlined. Electrophoretic mobility-shift assay was performed by incubating ³²P-labeled col2c2 (B) or HMG (C) probe with in vitro translation products derived from plasmids encoding no protein (pSG5), mouse SOX9, or zebrafish Sox9a or Sox9b followed by electrophoresis. A 100-fold excess of unlabeled oligo was added as competitor. “-” refers to samples with no competitors. Zebrafish Sox9a and Sox9b recognize the col2c2 motif and the HMG consensus sequence as effectively as does mouse SOX9. The protein–DNA complex can be competed away by unlabeled col2c2, but not by the unrelated kuc1. The unbound probe has run off the gel in this set of experiments.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 229, Chiang, E., Pai, C.-I., Wyatt, M., Yan, Y-L., Postlethwait, J., and Chung, B.-C., Two sox9 genes on duplicated zebrafish chromosomes: expression of similar transcription activators in distinct sites, 149-163, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.