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Fig. 4 Expression of zebrafish sox9a and sox9b in different adult tissues by RT-PCR. Total RNA from different tissues was used for RT-PCR analysis followed by hybridization to the sox9a or sox9b cDNA probe. Plasmids bearing sox9a and sox9b were used as control templates to monitor PCR fidelity and hybridization specificity. Actin primers were used to determine whether equal amounts of RNA were used for each PCR.
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Reprinted from Developmental Biology, 229, Chiang, E., Pai, C.-I., Wyatt, M., Yan, Y-L., Postlethwait, J., and Chung, B.-C., Two sox9 genes on duplicated zebrafish chromosomes: expression of similar transcription activators in distinct sites, 149-163, Copyright (2001) with permission from Elsevier. Full text @ Dev. Biol.