ZFIN Image: Choi et al., 2008, Fig. S4

Image

Figure Caption

Fig. S4 Anti-Sense Morpholino Oligos Specifically and Efficiently Knock Down Expression of Their Cognate Zebrafish miR-200 Targets
(A) Sequence alignments of synthesized antisense morpholino oligos directed against various miR-200 family members and their corresponding miR-200 family members. miR-141 MO is predicted to specifically hybridize to miR-141 and miR-200a, miR-200b MO is predicted to specifically hybridize to miR-200b and miR-200c and miR-429 MO is predicted to specifically hybridize to miR-420.
(B) One-cell embryos were injected with 4 ng of either miR-141 MO, miR-200b MO or miR-429 MO and systematically assayed for expression of miR-200 family members at 48 hpf indicating that the predictions made in (A) are valid.
(C) Wild type and fish injected with various morpholinos at 48 hpf are morphologically indistinguishable from each other.

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Expression / Labeling details

Acknowledgments

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