Fig. 5

Fig. 5 Fate mapping of rostral lateral plate mesoderm cells. (Animals are positioned with rostral to the left and dorsal up.) (A) White light image of illustrating the cells targeted for uncaging at 12 somites (arrowheads). e, eye; m, midbrain. Scale bar, 50 μm. (B) Video capture of a freshly labeled embryo at 13 somites, with uncaged cells shown in green by overlay from a fluorescent image. Arrowheads mark the caudal rim of the eye, asterisk marks the rostral end of the central nervous system. Scale bar, 100 μm. (C) The same embryo after the onset of circulation. The arrow marks the caudal rim of the eye, the asterisk the site of uncaging in the side of the head, and the arrowhead one of the large round fluorescent cells (left panel, white light image; middle panel, fluorescent image; right panel, merged image). Scale bar, 100 μm. (D) At higher magnification, a large round cell (arrow) is shown in the Ducts of Cuvier. The asterisk and arrow indicate the main channel in which red blood cells flow, but they cannot be seen in this image because they are moving too fast (left panel, white light image; middle panel, fluorescent image; right panel, merged image). Scale bar, 50 μm.