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Fig. 6 VP16-mbx expression inhibits eye and tectum formation. VP16-mbx mRNA (10 pg) together with β-galactosidase mRNA (300 pg), was injected into 1- to 2-cell-stage zebrafish embryos. (A, B) Live embryos at 25 hpf; injection of VP16-mbx caused a small eye phenotype (B). (C–F) In situ hybridization with mab21l2 (C), rx1 (D), and otx2 (E, F) at the 15-somite stage. Both mab21l2 and rx1 (purple staining) expression are smaller on the side that received the VP16-mbx mRNA injection (C, D), while otx2 expression is not affected (E). Red staining indicates the β-gal injection tracer.
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Reprinted from Developmental Biology, 248(1), Kawahara, A., Chien, C.-B., and Dawid, I.B., The homeobox gene mbx is involved in eye and tectum development, 107-117, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.