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Fig. 1 Sperm nuclei before (A, B) and after (C, D) demembranation. (A, C) Brightfield images, showing sperm heads on a hemacytometer. (B, D) Fluorescence images, showing Hoechst labeling of nuclei. Only a few nuclei are labeled prior to demembranation, whereas all nuclei are labeled after treatment with lysolecithin. Scale bar, 50 μm.
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Reprinted from Developmental Biology, 242(2), Jesuthasan, S. and Subburaju, S., Gene transfer into zebrafish by sperm nuclear transplantation, 88-95, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.