ZFIN ID: ZDB-IMAGE-080417-30
Figures for Kotani et al., 2008

Figure Caption/Comments:

Fig. 3 Rescue of the mys mutant phenotype by transgenesis. (A) The structure of the Tol2–mys construct. mys cDNA (mys) was cloned between the Xenopus EF1α promoter (EF1α-p) and the SV40 polyA signal (poly A). Thick black lines indicate the Tol2 sequence. (B) A transposon-donor plasmid containing Tol2–mys and the transposase mRNA were co-injected into fertilized eggs produced from mys homozygous parents. The injected fish were crossed with mys homozygous fish and transgenic fish were identified in the progeny. A female homozygous for the mys mutation (mys/mys) and carrying a single copy insertion of Tol2–mys was crossed with a wild type male, and the progeny were analyzed for the phenotype. (C, D) Dorsal views of embryos at the 6-somite stage. (C) Embryos from homozygous (mys/mys) female fish showed the obscure somite boundary phenotype. (D) Embryos from mys/mys; Tg(Tol2–mys) female fish showed distinct somite boundaries.

Figure Data:
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.