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Fig. 3 The chronic effects of GFP::Mdm2 expression in Gmdm2-liver larvae and adult fish resulting in developmental delay and growth retardation. A-F: G-liver and Gmdm2 larvae and their growth were assessed at 14, 50, and 100 days post-fertilization (dpf). Representative pictures of the fish are shown. Gmdm2-liver fish are significantly shorter and thinner than G-liver fish until 100 dpf. G: Genetic statistical analysis of developmental delay and growth retardation in two Gmdm2-liver lines compared with G-liver and wild-type fish. H: Severe growth retardation of two 10-month-old Gmdm2-liver fish lines compared with G-liver and wild-type fish. Insets show higher magnification images of the cross-sections of zebrafish liver at various stages. Scale bars = 200 μm (A,D); 10 mm (B,E); 1 cm (C,F,H).