ZFIN Image: Raschperger et al., 2008, Fig. 2

Image

Figure Caption

Fig. 2 CAR protein expression in zebrafish pronephros. Immunohistochemical co-stainings of CAR with the tight junctional protein ZO-1 were performed on transversal sections of the pronephros from a 96 hpf zebrafish larvae. (A) CAR and ZO-1 were detected in parietal epithelium (pe) of the Bowman's capsule that surrounds the pronephric glomerulus (pg) and in the gut (g). Podocyte and endothelial expression of ZO-1 was also detected in the pronephric glomerulus. (B) In the pronephric tubules (pt), CAR and ZO-1 co-localize to epithelial cell–cell contacts close to the lumen (asterisks in panels B and C), and this apical localization was verified by co-staining of CAR and the basolateral marker Na⁺/K⁺-ATPase alpha1 subunit (C). The notochord (n) is indicated in panel A for orientation and DAPI was used to visualize the nuclei (blue). Scale bar, 50 μm.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 313(1), Raschperger, E., Neve, E.P., Wernerson, A., Hultenby, K., Pettersson, R.F., and Majumdar, A., The coxsackie and adenovirus receptor (CAR) is required for renal epithelial differentiation within the zebrafish pronephros, 455-464, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.