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Fig. 2

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ZDB-IMAGE-080325-2
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Figures for Sperber et al., 2008
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Fig. 2 dlx1a and dlx2a are required for proper head outgrowth and arch cartilage patterning. (A–D) Lateral views of head morphology in 5-day-old larvae that received the (A) control MO, 0.5 mM; (B) dlx1aMO, 0.5 mM; (C) dlx2aMO, 0.25 mM; (D) an equal combination of dlx1aMO + dlx2aMO 0.25 mM each. (E–P) Cartilage elements stained with alcian blue. (E–H) are lateral views; (I–L) are ventral view. The inset in panel H is a lateral oblique view showing loss of the interhyal by fusion of hyosymplectic and ceratohyal elements (arrowhead). (M–P) Dissected ceratobranchial arch elements, numbered. cb, ceratobranchials; ch, ceratohyal; eth, ethmoid plate; hys, hyosymplectic; ih, interhyal; M, Meckel′s cartilage; pq, palatoquadrate. Arrows (A–D) indicate position of the mouth; asterisk in panel P indicates site of lateral branching. (A–P) Anterior is to the left. Scale bar in panel A: 250 µm; panels E and I: 100 μm; panel H inset, and M: 50 μm.

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Reprinted from Developmental Biology, 314(1), Sperber, S.M., Saxena, V., Hatch, G., and Ekker, M., Zebrafish dlx2a contributes to hindbrain neural crest survival, is necessary for differentiation of sensory ganglia and functions with dlx1a in maturation of the arch cartilage elements, 59-70, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.