IMAGE

Fig. 6

ID
ZDB-IMAGE-080324-10
Source
Figures for Sullivan-Brown et al., 2008
Image
Figure Caption

Fig. 6 Na+/K+ATPase localization is disrupted in cystic tissues, while apical polarity is maintained. Apical localization of F-actin (red) observed in 2 dpf wild-type siblings (A) is maintained in the medial tubules (white arrowheads) of mutants (B–D). The somites and gut are also stained by phalloidin (red). Correct apical localization of ZO-1 (green) is observed in 4 dpf wild-type sibling (E) and mutant (F–H) embryos. Sibling tubules are outlined by a white circle, and mutant tubules are distinguished by asterisks. Basolateral localization of the Na+/K+ATPase (red) observed in wild-type siblings (I) is altered in cystic mutants at 4 dpf (J–L). Note in panels J–L that Na+/K+ATPase is adjacent to apical ZO-1 expression (green; indicated by white arrows) which is not observed in wild-type (I). All confocal images were taken with a 40× water objective lens. Panels A–D are cryosections; panels E–L are plastic sections. Nuclei (blue) are stained with either DRAQ5 (A–D) or Hoechst (E–L). ZO-1 staining basal to the pronephros comes from a different tissue (asterisks in panels K and L) and does not reflect an alteration in ZO-1 within the pronephros.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 314(2), Sullivan-Brown, J., Schottenfeld, J., Okabe, N., Hostetter, C.L., Serluca, F.C., Thiberge, S.Y., and Burdine, R.D., Zebrafish mutations affecting cilia motility share similar cystic phenotypes and suggest a mechanism of cyst formation that differs from pkd2 morphants, 261-275, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.