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Fig. S2

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ZDB-IMAGE-071228-11
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Figures for Veldman et al., 2007
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Fig. S2 Morpholino specificity demonstrated by knockdown of GFP reporter expression or transcript mis-splicing in morpholino-treated embryos. Single cell zebrafish embryos were injected with morpholino, rhodamine dextran, and a GFP reporter plasmid and assayed for GFP expression 24 h later. (A and B) Bright-field images of 24 hpf embryos injected with control morpholino (C-MO) (A) or SOCS3a targeting morpholino (B) plus SOCS3a-GFP reporter plasmid. (C and D) Red fluorescence from rhodamine dextran demonstrating efficient injection in both embryos. (E and F) GFP expression is present in many cells of the C-MO-injected embryo (arrows in E) while GFP is absent in the target morpholino injected embryo (F). Auto-fluorescence present in the yolk is denoted by an asterisk. (G) Tabular results of embryo counts for each reporter and morpholino used in this study. In all cases, the C-MO had little effect on reporter expression while each targeting morpholino significantly decreased the number of GFP-positive embryos. (H) Single cell embryos were injected with control (C) or KLF splice-site targeted (SP) morpholino and RNA isolated 24 h later for analysis of mis-splicing using RT-PCR. Note that the splice site targeted morpholinos resulted in mis-splicing of each gene with mis-splicing of KLF7a being complete and mis-splicing of KLF6a being partial.

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Reprinted from Developmental Biology, 312(2), Veldman, M.B., Bemben, M.A., Thompson, R.C., and Goldman, D., Gene expression analysis of zebrafish retinal ganglion cells during optic nerve regeneration identifies KLF6a and KLF7a as important regulators of axon regeneration, 596-612, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.