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Fig. 4 olig2+ radial glial cells have asymmetric features. A-C: Transverse sections of 15-dpf Tg(olig2:egfp) fish, dorsal up. A: aPKC (red) was localized to the apical membranes of EGFP+ radial glia (green) at the central canal. B: Two BrdU+ EGFP+ cells (arrows) were next to each other near the central canal. These cells might have been recently divided siblings. C: Anti-phospho-Histone H3 (pH3) antibody labeling revealed chromosomes (arrows) aligned with the axis of division perpendicular to the central canal (dashed line). Scale bar = 10 μM.
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