ZFIN Image: Wang et al., 2007, Fig. 5

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Figure Caption

Fig. 5 Induction of p21 through PI3K/Akt is involved in the survival signaling of NRP-1. The activation of NRP-1/EGNP-1 increased p21 expression. EGNP-1 transduced HUVEC or PAEC/NRP-1 were subjected to EGF or VEGF for the indicated times and their lysates were immunoblotted for p21 with PLC-γ as a loading control. b. The activation of EGNP-1 induced p21 phosphorylation at its Tyr¹⁴⁵ and Ser¹⁴⁶ sites. Immunoblotting was performed with anti-p21Tyr and anti-p21Ser in EGNP-1 transduced HUVEC stimulated by EGF for the indicated times. c. Akt mediates the phosphorylation of p21 in EGNP-1-mediated signaling. HUVEC were transfected with EGNP-1 then treated with Ly294002 or co-transfected with EGNP-1 and Akt(DN). Immunoblotting was performed to quantify the expression and activation of p21. p21 and phospho-p21 were quantified as a ratio to the amount of PLC-γ. *p<0.001, **p<0.005, ***p<0.01, ****p<0.05 in a Student's t test.

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