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Fig. 1 Inca is a novel Tfap2a target in the NC. (A) Animal caps were injected with Wnt3a, Chd and GR-AP2a mRNA, treated with 10 μM dexamethasone (DEX+) or solvent alone (DEX-), and subjected to northern blot analysis using probes as indicated, with 18S rRNA as a loading control. UI, uninjected. (B) Predicted protein sequence alignment for Xenopus (X), zebrafish (Z), mouse (M) and human (H) Incas. Identical residues are shaded. The conserved 38-residue inca-box is underlined, and the 14-3-3 binding site is indicated with star underline. (C) Dendrogram generated using ClustalW (http://clustalw.genome.jp). Accession Numbers are: Xenopus IncaA, BJ092564. Mouse, AK076092. Human, NM_203370. Zebrafish inca1, CK018555; inca2, BM071087. Human Inca-r, BC031558. Mouse Inca-r, NM_175398. Zebrafish inca-r, BI885065. Xenopus (tropicalis) Inca-r, CR761080. chr, chromosome number.