ZFIN Image: Kwan et al., 2007, Fig. 2

Image

Figure Caption

Fig. 2 Validation of reporters and internal ribosome entry sequence (IRES) constructs. Embryos shown were injected at the one-cell stage with expression constructs made with the pISce-Dest destination vector, then mounted at 24 hours postfertilization (hpf) for confocal microscopy of the trunk. All plasmids tested generate functional bicistronic messages, as demonstrated by the presence of both mCherry and EGFP fluorescence. A-A″:bactin2: nlsmCherry-IRES-EGFPCAAXpA. B-B″:h2afx: nlsmCherry-IRES-EGFPCAAXpA. C-C″:bactin2: mCherry-IRES-EGFPCAAXpA. D-D″:bactin2: nlsmCherry-IRES-EGFPpA. E-E″:bactin2: mCherryCAAX-IRES-nlsEGFPpA. F:bactin2: nlsEGFP-IRES-EGFPCAAXpA. G:bactin2: EGFPCAAX-IRES-nlsEGFPpA. Scale bar = 50 μm.

Acknowledgments

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