ZFIN Image: Iovine et al., 2005, Fig. 3

Image

Figure Caption

Fig. 3 Expression of connexin 43 in embryos. In situ hybridization was completed using a cx43 probe against the cx43 3′ UTR. (A) Expression of cx43 in wild-type embryo at 24 hpf. e, eyes; c, cerebellum; v, vasculature. (B) Expression of cx43 in sof embryo at 24 hpf. e, eyes; c, cerebellum; v, vasculature. (C) Expression of cx43 in wild-type larvae at 72 hpf. Embryos were treated with PTU to prevent the production of melanin and facilitate the identification of stained structures. l, lens epithelium; h, hindbrain; t and arrowhead, thymus. (D) RT-PCR from 72 hpf whole embryos (lane 1), 72 hpf embryonic hearts (lane 2), and adult hearts (lane 3). The top panel shows amplification from cx43-3′ UTR primers, the bottom panel shows amplification from col-1a primers. Approximate sizes of amplified products are shown on the right (arrowhead).

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 278(1), Iovine, M.K., Higgins, E.P., Hindes, A., Coblitz, B., and Johnson, S.L., Mutations in connexin43 (GJA1) perturb bone growth in zebrafish fins, 208-219, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.