ZFIN Image: Zhu et al., 2005, Fig. 3

Image

Figure Caption

Fig. 3 Transplantation of primitive progenitors into vlad tepes (vlt) recipients. (A) The EGFP+ population from 10- to 12-somite LGb; Tg(gata1:DsRed) transgenic embryos was isolated by FACS to 90% purity and transplanted into 48 hpf vlt embryos. (B) One day after transplantation, circulating donor-derived cells were identified by DsRed and EGFP fluorescence. By 3 days post transplantation, most circulating cells were DsRed⁺, suggesting that the EGFP+ donor progenitors had differentiated into Gata1⁺ erythrocytes (arrow). One month after transplantation, each of the 8 survivors carried approximately 10–200 DsRed+ circulating cells (arrows). At 1 month, we also observed EGFP expression in the trunk vasculature (arrowheads) in 2 of 8 recipients. A table summarizing the results is shown in C.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 281(2), Zhu, H., Traver, D., Davidson, A.J., Dibiase, A., Thisse, C., Thisse, B., Nimer, S., and Zon, L.I., Regulation of the lmo2 promoter during hematopoietic and vascular development in zebrafish, 256-269, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.