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Fig. 3

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ZDB-IMAGE-070920-14
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Figures for Christie et al., 2006
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Fig. 3 Expression of zebrafish CRMP2 from the 12- to 20-somite stages. At the 12-somite stage, CRMP2 is expressed at low levels ubiquitously throughout the embryo in both neural and non-neural tissue (A) as compared to sense controls (C). Slightly higher levels of expression are detected in the anterior portion of the embryo (A) as well as in regions just lateral to, and within, the hindbrain corresponding to the developing trigeminal sensory ganglia (TG/tg) and hindbrain clusters (asterisk, B) respectively. At the15 somite stage CRMP2 continues to be detected in the TG (D and E) and future hindbrain clusters (asterisk, E). CRMP2 is also expressed in cells of the dorsal rostral cluster (drc) of the telencephalon, ventral rostral cluster (vrc) of the diencephalon (D), and Rohon Beard cells (RB) of the neural tube (box in D, enlarged dorsal view in F). The expression pattern of CRMP2 in the TG and RB cells is compared to that of Zn-12, an antibody recognizing both types of cells (G, H, I). By the 20 somite stage CRMP2 is now also detected in the ventral caudal cluster (vcc) of the mesencephalon (J). Expression of CRMP2 in these major clusters and ganglia can be seen in cross-sections (L–N). At the 20-somite stage, expression of CRMP2 overlaps with PlexinA4 expression in the TG (K versus O). All whole-mount embryos are presented anterior to the left. Figures B, E, F, H, I, K and O are de-yolked, dorsal views. Figures L, M, and N are 7 μm sections taken through the regions of the brain as outlined in J and K.

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Reprinted from Gene expression patterns : GEP, 6(2), Christie, T.L., Starovic-Subota, O., and Childs, S., Zebrafish collapsin response mediator protein (CRMP)-2 is expressed in developing neurons, 193-200, Copyright (2006) with permission from Elsevier. Full text @ Gene Expr. Patterns