Fig. 6

Fig. 6  Immunohistochemical detection of Mgp accumulation in zebrafish (A–I) and sole (J–L). (A) Accumulation of Mgp in the otolith at 6 dpf (400×) and (B) in the otolith and cleithrum at 8 dpf (400×); (C) Consecutive section (to that in B) of the otolith (arrowhead) and cleithrum (arrow) stained by Alizarin red and counterstained with toluidine blue (400×); (D) accumulation of Mgp at the zones initiating mineralization of neural arches in pleural vertebrae 3–5 of a 13 dpf larva (1000×); (E) accumulation of Mgp in the mineralizing branchial arches and pharyngeal teeth at 13 dpf; (F) consecutive section (to that in E) stained with von Kossa’s; (G) Mgp accumulation in the forming caudal vertebra and associated arches at 20 dpf (250×); (H) Mgp accumulation in the preopercular bones under endochondral ossification (arrows) in a 40 dpf juvenile (1000×); (I) Mgp accumulation in skull bones undergoing intramembranous ossification (arrow) at 40 dpf (1000×); (J) Mgp accumulation in the otic capsule (OC) at 8 dpf (200×); (K) Mgp accumulation in the mineralizing matrix under the trabecula (T) of a 17 dpf larva (400×); (L) Mgp accumulation in the mineralizing branchial arches and pharyngeal teeth of a 26 dpf (200×), but also in the aorta and cardiac arterial bulbus (arrowhead). No staining was observed in control sections incubated with pre immune serum (results not shown).