Fig. 2

Fig. 2 Sarcomeric proteins are mislocalized in ruz mutant skeletal muscle. Indirect immunofluorescence was performed on longitudinal sections of 5–6 dpf mutant and wild-type skeletal muscle and samples were imaged at 100× (A, B) or 400x (C–F). (A, C) β-Dystroglycan protein localizes to the myosepta of wild-type embryonic muscle. (B, D) In ruz mutants, β-dystroglycan is maintained at the myosepta. (C) Nebulin localizes to the sarcomere in wild-type embryos. (D) Nebulin sarcomeric localization is lost throughout most of ruz mutant muscle. (E) Skeletal muscle actin similarly localizes to the sarcomere in wild-type embryos while localization is lost in ruz mutants (F). Nuclei were stained with DAPI (blue).