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Fig. 6 mef2ca is autonomously required in cranial neural crest for skeletal patterning. Wild-type rhodamine-labeled fli1:EGFP CNC cells were unilaterally transplanted into mef2ca mutant hosts. (A–B) Confocal micrographs of mef2ca mutant with wild-type CNC mosaic fish from the transplanted side (A) and control side (B). On the transplanted side, lineage tracer is detected throughout dorsal and ventral cartilages, as well as joint regions (arrows). On the control side, only mef2ca mutant host fli1:GFP CNC derivatives are seen. (C) Flatmounted pharyngeal skeleton, double stained for cartilage in blue and bone in red. Dorsal/ventral cartilage joints have been rescued on the transplanted side (arrows) but not on the control side (asterisks). Opercle and branchiostegal ray morphology is also completely rescued on the transplanted side, but not the control side. bsr, branchiostegal ray; op, opercle. Scale bars: 100 μM.
Reprinted from Developmental Biology, 308(1), Miller, C.T., Swartz, M.E., Khuu, P.A., Walker, M.B., Eberhart, J.K., and Kimmel, C.B., mef2ca is required in cranial neural crest to effect Endothelin1 signaling in zebrafish, 144-157, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.