Fig. 5

Fig. 5 ndr2 expression in ntl and flh mutant embryos. The left column shows wild-type siblings, the right column shows ntl homozygotes (B, D, F, H, J) or a flh homozygote (L). (A–F) Bud stage. (A, B) Lateral view, anterior to the left. (C, D) Anterior view. ndr2 is expressed in anterior notochord and prechordal plate. The embryonic stage shown here is slightly earlier than that shown in Figs. 4F and 4G, and therefore the gap within the anterior expression domain is not yet evident. ndr2 expression is slightly wider in the ntl embryo. (E, F) Posterior view. ndr2 staining is found in the tail bud of wild-type siblings (arrow), while in mutant embryos, tail bud staining is absent but a broader lateral and posterior region is positive (asterisk); 16 (24%) of 66 embryos in a cross between heterozygous ntl zebrafish showed the mutant pattern. (G–L) 20-somite stage. In wild-type embryos, ndr2 is expressed asymmetrically in the lateral plate and diencephalon, but expression is present bilaterally in both ntl (H, J) and flh mutants (L).