IMAGE

Fig. 5

ID
ZDB-IMAGE-061110-20
Source
Figures for Pineda et al., 2006
Image
Figure Caption

Fig. 5 Ventrally projecting motor axons displayed abnormal trajectories in 1.6MO morphants even though CaP axons project normally. (A-D) Motor axon morphology was assayed using SV-2 immunoreactivity (green) in conjunction with a marker of the muscle postsynaptic receptor, α-bungarotoxin (red). At 48 hpf (A,B), little difference was noted in znp-1 immunoreactivity of motor axons of 1.6MO morphants (B) compared with control-injected embryos (A). However, by 72 hpf, there were marked differences (C,D). Motor axons of 1.6MO morphants (D, asterisks) branched more than those of control-injected embryos. Moreover, in morphants (D), there was a reduction in the normal alignment between motor axons and postsynaptic receptors, assessed by the near absence of green and abundance of yellow in C versus the reduction in yellow and increase in green in panel D. (E-L) Injection of 1.6MO did not affect axon outgrowth from CaP when synapses first form. (E-H) At 21-22 hpf, znp-1 immunoreactivity revealed no differences between ventrally projecting CaP axons in either control (E,F) or 1.6MO-injected (G,H) embryos. Embryos were squash-mounted and, consequently, motor nerves on both sides of an embryo were often present in a single confocal section (e.g. F,H). (I,J) CaPs were directly labeled by dye injection (green) at 20 hpf and embryos were fixed at 21-22 hpf and stained for SV-2 immunoreactivity. Injection of either ConMO (I) or 1.6MO (J) did not affect CaP axon morphology. (K,L) In addition to axonal morphology (SV-2, green), postsynaptic specializations, as detected by α-bungarotoxin labeling (red) were not altered by injection of either Con (K) or 1.6 (L) MO. Scale bars: in A, 20 μm for A,B; 30 μm for C; in E, 50 μm for E-H; in J, 10 μm for I,J; in L, 20 μm for K,L.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development