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Fig. 1 The development of dbh-positive cervical sympathetic ganglia (CSG) is severely affected in hands off embryos. Whole-mount in situ hybridization for dbh in wild-type (A,B) and hands off mutant embryos C,D) at 2 dpf (A,C) and 3 dpf (B,D). dbh-positive CSG cells (arrowheads) are missing in hands off embryos, whereas dbh-positive cells in the dorsally located medulla oblongata are not affected [asterisks; noradrenergic neurons of the medulla oblongata/area postrema form a semicircle (Holzschuh et al., 2003), giving the impression of two stripes in the oblique view from lateral shown].
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