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Fig. 5 Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of Tnnt2 alternatively spliced variants during zebrafish development. A: Schematic representation of the positions of the primers used to perform RT-PCR. Empty boxes indicate untranslated regions; solid boxes show open reading frames. B: Age-dependent expression of alternatively spliced, Tnnt2 variants. Alternatively spliced variants were detected by RT-PCR using the primer pair combinations indicated. Two, alternatively spliced variants were detected by primer pair Tnnt2-F and Tnnt2-R3 in adult fish. C: Schematic illustration of the alternative splicing involved in the generation of Tnnt2 (unspliced form), Tnnt2-6E (excluding exon 6), and Tnnt2-4E (excluding exon 4). The alternatively spliced exons are underlined.